What is PromPTTM?
PromPTTM is a bioinformatics platform and library-focused DNA construction technology that allows Synpromics to design, build and test synthetic promoters that regulate gene expression in a highly specific and machine driven manner. Using this platform, Synpromics has created promoters that only control expression in particular cell types, or in response to environmental, biological or chemical stimuli.
How does it work?
We assess the gene expression profile of the target cell and condition by identifying differentially expressed genes using microarray or NGS technology. We then employ a number of proprietary bioinformatics scripts and algorithms to identify the enhancer elements that control the expression of those genes. We integrate data from a diverse array of sources in order to identify these enhancer regions and feed that data into the customised genome browser that we have developed. This allows us to integrate data from a vast array of sources, either publicly available or from functional genomics data we generate in house.
We then use novel library screening technology to resolve the precise enhancer sequences that control transcription and use these sequences as parts to build synthetic promoters using a rational engineering biology approach. The pipeline generates novel promoters that tightly control gene expression in the particular cell type and condition of interest, and comprises a sequence constituting a novel combination of enhancer elements that does not exist in nature and thus can be patented. Underlying all the above stages are our custom designed Synpromics Promoter database and Design platform, which capture and record all of the design process and allows our diverse scientific team to combine powerful machine learning techniques with human knowledge and instinct.
The databases that form part of PromPTTM are some of the most comprehensive resources for the understanding of gene regulation that have been developed to date, and enable us to identify new regulatory sequences from genomes with a higher degree of accuracy. This allows us to take a unique approach to promoter design and construction compared to our competitors.
Most other organizations working on gene control are using natural promoter systems that are not optimally designed for the task at hand. We take a novel engineering biology approach and build completely novel promoters and expression cassettes that are specifically designed to function optimally in the specified application.
Simply, our technology allows the expression of the gene of interest in a highly specific and controllable fashion.
For Cell and Gene Therapy applications, the ability to transcriptionally target the gene of interest allows an extra layer of safety, preventing off-target expression and reducing potential side effects of the treatment. Moreover, inducible promoters allow gene expression to be switched off if a subsequent pathological condition develops and enables tunable expression of the therapeutic gene in situ, so that expression of the gene can increased or decreased depending on the circumstance.
We are also able to produce very powerful promoters that could allow viral vector doses to be reduced and hence improve the safety profile and longevity of expression of the therapy.
For Bioprocessing applications, we can create promoters that function optimally in a particular manufacturing cell line, or that are designed to regulate the expression of a specific class of protein, e.g. monoclonal antibodies. Moreover, the panels of promoters that we create mediate a range of different expression levels, so we can choose different synthetic promoters to titrate the expression of multiple different protein components in order to achieve the optimal ratio and production of protein, i.e. the heavy and light chains of an antibody for instance.