Synpromics' promoters drive protein expression in mammalian cell manufacturing systems mediating improved stability and enhanced expression compared to the current industry standards.
Our promoters can be designed for constitutive or inducible gene expression in any eukaryotic system, enhancing protein levels to further improve biopharmaceutical manufacturing processes and produce protein with the correct post-translational modifications, ultimately reducing production costs. We can select candidates from existing promoter libraries or engage in a design and development program to deliver promoters with particular criteria that are unique to the specific application.
Production of Simple or Complex Protein Products
Synthetic promoters produced by Synpromics can be used to drive expression of a single protein product, or in the context of a more complex biological production system, i.e. where multiple genes need to be expressed to yield a biological product, such as genes encoding the different subunits of monoclonal antibodies. The main advantage of using multiple unique synthetic promoters to drive expression of multiple genes is that the lack of sequence homology between promoters prevents homologous recombination and shutdown of expression.
Stable and High Expression
Synpromic’s design platform has enabled the creation of a suite of constitutive promoters for use in bioprocessing and viral vector manufacturing. This suite of promoters has a 50-fold dynamic range, from many times stronger than commonly used viral promoters to very weak. This large range of promoters allows for the fine-tuning of expression level to obtain the optimal yield from the manufacturing process.
By designing promoters that are customised for a particular cell type, e.g. CHO, HEK293, or other proprietary cell lines, we have shown that it is possible to obtain higher and more predictable levels of protein expression compared to natural promoters or common viral promoters, such as the cytomegalovirus (CMV) promoter.
Adaptable to Pathway Engineering
Novel assemblies of enhancer elements can also be used to edit natural endogenous gene promoters in the genome using new editing techniques in order to modulate the expression of endogenous genes and engineering more effective producer cells by pathway optimisation to enhance protein folding and/or processing. Multiple genes can be targeted using this approach, where different pathway components are required to be expressed at different levels, by using distinct enhancer element assemblies with unique sequences.
Inducible Expression in Any Environment
Synpromic’s design platform allows the creation of strong, highly responsive and tightly regulated promoters controlled by either addition of a safe small molecule or via changes in the cellular environment. These promoters allow the development of new processes for traditionally problematic proteins/viruses and provide the tools to overcome the bottlenecks in current bioprocessing and viral vector manufacturing.
Synpromics is able to create promoters that are responsive to any change of the environment of the producer cell, such as an environmental change of temperature, a physico-chemical change of pH or the addition of any biological or chemical stimuli. This allows the producer cell line to reach a certain biomass before production initiates and facilitates the production of toxic or difficult to express proteins.
Our novel approach to synthetic promoter development means that it is now possible to develop new promoters to tackle the biotechnology challenges of biologics manufacturing. We can either select and out-license candidates from existing promoter libraries or alternatively engage in a design and development program to deliver promoters with particular criteria that are unique to the specific application