What is PromPT®?
PromPT® is a suite of bioinformatics tools and library-focussed DNA assembly techniques that allows Synpromics to design, build and test synthetic promoters that regulate gene expression in a highly specific manner. Using this platform, Synpromics have created promoters that only control expression in particular cell types, or in response to environmental, biological or chemical stimuli.
How does it work?
We assess the gene expression profile of the target cell and condition by identifying differentially expressed genes using microarray or NGS technology. We then employ a number of proprietary bioinformatics scripts and algorithms to identify the enhancer elements that control the expression of those genes. We integrate data from a diverse array of sources in order to identify these enhancer regions and feed that data into the customised genome browser that we have developed. This allows us to integrate data from a vast array of sources, either publicly available or from functional genomics data we generate in house.
We then use novel library screening technology to resolve the precise enhancer sequences that control transcription and use these sequences as parts to build synthetic promoters using a rational engineering biology approach. The pipeline generates novel promoters that tightly control gene expression in the particular cell type and condition of interest, and comprises a sequence constituting a novel combination of enhancer elements that does not exist in nature and thus can be patented.
The databases that form part of PromPT® are some of the most comprehensive resources for the understanding of gene regulation that have been developed to date, and enable us to identify new regulatory sequences from genomes with a higher degree of accuracy. This allows us to take a unique approach to promoter design and construction compared to our competitors.
Most other organisations working on gene control are using natural promoter systems that are not optimally designed for the task at hand. We take a novel engineering biology approach and build completely novel promoters and expression cassettes that are specifically designed to function optimally in the specific application that our partners are interested in.
Simply, our technology allows customers to express their gene of interest in a highly controllable fashion.
From a cell and gene therapy perspective, the ability to transcriptionally target the gene of interest allows an extra layer of safety, preventing off-target expression and reducing potential side effects of the treatment. Moreover, inducible promoters allow gene expression to be switched off if a subsequent pathological condition develops and enables tunable expression of the therapeutic gene in situ, so that expression of the gene can increased or decreased depending on the circumstance.
We are also able to produce very powerful promoters that could allow viral vector doses to be reduced and hence improve the safety profile and longevity of expression of the therapy.
For Bioprocessing applications, we can create promoters that function optimally in a particular manufacturing cell line, or that are designed to regulate the expression of a specific class of protein, e.g. monoclonal antibodies. Moreover, the panels of promoters that we create mediate a range of different expression levels, so our partners can choose different synthetic promoters to titrate the expression of multiple different protein components in order to achieve the optimal ratio and production of protein, i.e. the heavy and light chains of an antibody for instance.
Also, our inducible promoters allow manufacturers to achieve a particular biomass in the bioreactor before turning on expression of the target protein and hence optimise yield.
Contact us to find out more about PromPT® and our partnering opportunities.