There is a need in the industry for systems that test for biological activity and/or biological safety using methods which are not only highly predictive of actual effect on the human body, but are also convenient in use, easily transferable and standardisable in practice. The need to test for therapeutic value, health benefit or biological safety is common to many industry sectors and:
- Has obvious use in conventional drug discovery
- Is similarly applicable in the growing field of natural product therapeutics
- Is finding new applications in testing of nutraceuticals and food supplements
- Has clear potential to provide means of evaluating the biosafety of nanostructured materials increasingly present in industrial and domestic environments.
In all cases, the most convenient and ethically acceptable way to do this is by testing on living human cells in culture, i.e. cell-based analysis. This offers potential for reiterative testing, for low-cost rapid throughput compared with animal models or clinical trials, and standardisation of materials and protocols across testers. Growing industry recognition that cell-based analysis may lack predictive power - due to aberrant behaviour of aged cell lines on which most assays have hitherto been based - has stimulated the adoption of primary or stem cell-based assays.
Next Generation Cell-Based Assays
Synpromics' technology can be adapted for bioassay development in order to:
- Customise cell lines harboring promoters responsive to biological stimuli
- Develop assays based on high-throughput protocols
- Edit cell lines with a view to increasing sensitivity to biological stimuli
- Customise potency assays responsive to the desired stimuli
Synpromics' promoters offer tight control of gene expression, and the development inducible promoters that are incorporated into cell-based assays can be tailored to stimuli such as:
- Pathogen infection
- Disease induction
Developed promoters comprising short unique DNA sequences are ideally suited for multiplexing, i.e. multiple readouts in a single cell, and can also be used for animal model development.