Our promoters can be designed for constitutive or inducible gene expression in any eukaryotic system, enhancing protein levels to further improve biopharmaceutical manufacturing processes and produce protein with the correct post-translational modifications, ultimately reducing production costs.
Production of Simple or Complex Protein Products
Synthetic promoters produced by Synpromics can be used to drive expression of a single protein product, or in the context of a more complex biological production system, i.e. where multiple genes need to be expressed to yield a biological product, such as genes encoding the different subunits of monoclonal antibodies. The main advantage of using multiple unique synthetic promoters to drive expression of multiple genes is that the lack of sequence homology between promoters prevents homologous recombination and shutdown of expression.
Stable and High Expression
By designing promoters that are customised for a particular cell type, e.g. CHO, HEK293, proprietary cell lines, or a particular yeast strain, e.g. Pichia pastoris, Sacchromyces cerevisiae, we have shown that it is possible to obtain higher and more predictable levels of protein expression compared to natural promoters or common viral promoters, such as the cytomegalovirus (CMV) promoter.
Adaptable to Pathway Engineering
Novel assemblies of enhancer elements can also be used to edit natural endogenous gene promoters in the genome using new editing techniques in order to: modulate the expression of endogenous genes; and engineer more effective producer cells, through pathway optimisation with a view to enhancing protein folding and processing. Multiple genes can be targeted using this approach, where different pathway components are required to be expressed at different levels, by using distinct enhancer element assemblies with unique sequences.
Inducible Expression in Any Environment
Finally, Synpromics is able to create promoters that are responsive to any change of the environment of the producer cell, such as an environmental change of temperature, a physico-chemical change of pH or the addition of any biological or chemical stimuli. This allows the producer cell line to reach a certain biomass before production initiates and facilitates the production of toxic or difficult to express proteins.